Enter what general purpose this chemical will be having in this laboratory. Identification sodium borohydride is a white, odorless powder or pellet. Sodium cyanoborohydride msds material safety data sheet or sds, coa and coq, dossiers. Sodium cyanoborohydride is especially suitable for reductive aminations. Sodium cyanoborohydride 10g is dissolved in thf 80ml and 1 m methanolic hydrochloric acid is added until ph reaches 9. Do not use mouthtomouth method if victim ingested or inhaled the substance. Amount of sample from 25 pmol up to 25 nmol glycans per sample. Sodium borohydride revision date 17jan2018 attention is required. Sodium cyanoborohydride mda reaction conditions, hive. Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910. Sodium cyanoborohydride reagent grade american elements.
Reductive amination of mdp2p using sodium cyanoborohydride. Firstaid measures general advice immediate medical attention is required. It is used for bleaching wood pulp, as a blowing agent for plastics, and as a reducing agent for aldehydes and ketones. Detection method for sodium cyanoborohydride organic. View product specific information, msds, references and buying faq. The bound cyanoborohydride can be utilized as a versatile reducing agent for the reductive amination of carbonyl compounds and reduction of. Sigmaaldrich material safety data sheet date printed.
Tm 2aa 2aminobenzoic acid monosaccharide release and. In normal use conditions and in its original form, the product itself does not involve any other risk for health and the environment. I need to find a method to analyse the clearance of sodium cyanoborohydride throughout a process we believe it has been cleared but we are strugglinf to find an assay that will show this with high sensitivity. Department of chemistry, university of minnesota, minneapolis, minnesota 55455.
Sodium borohydride, also known as sodium tetrahydridoborate and sodium tetrahydroborate, is an inorganic compound with the formula na bh 4. According to the material safety data sheet msds special precautions must be taken when working with the chemical described above. Pa 800 plus pharmaceutical analysis system carbohydrate. Sodium cyanoborohydride solution american elements. Download msds pdf search msds currently available for download bioworld offers sodium cyanoborohydride for your research at low price. Thermo scientific aminolink reductant sodium cyanoborohydride promotes the formation of stable bonds between aldehyde and aminecontaining molecules, enabling efficient labeling, conjugation, and immobilization of proteins and other molecules.
Canadian ingredient disclosure list section 16 additional information msds creation date. Sodium borohydride can affect you when breathed in and by passing through your skin. Pierce coimmunoprecipitation coip kit thermo fisher scientific. Sodium cyanoborohydride revision date 19january2018 eye contact in the case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Apr 01, 1982 reductive methylation of protein amino groups with formaldehyde and sodium cyanoborohydride is shown to give up to 25% yield of ncyanomethyl ch2cn product. Msds contains all of the information required by those regulations. Does anyone have any suggestions on what could be used. This white solid, usually encountered as a powder, is a reducing agent that finds application in chemistry, both in the laboratory and on an industrial scale. The precipitate is collected and stirred for 2 h in ethyl acetate 250ml.
Sds of sodium cyanoborohydride, safety data sheets, cas 25895. It has been tested as pretreatment for pulping of wood, but is too costly to be commercialized. Lane marker sample buffer, nonreducing, 5x, 5ml, 0. Buy sodium cyanoborohydride cas 25895607, a highly selective reducing agent for reductive amination, from santa cruz. Sodium cyanoborohydride is the chemical compound with the formula nabh3cn. The cyanoborohydride offers five times milder reduction compared to borohydride in the reductive amination process, reducing the schiff s. Sodium borohydride msds material safety data sheet or sds, coa and coq, dossiers. Firstly a summary of the preparation and properties of sodium cyanoborohydride is given. Samples of the sodium cyanoborohydride prepared in examples 1114 and a sample of sodium cyanoborohydride prepared in accordance with the general teachings of u. Examples include dna purification procedures, preservation of biological material, and as a solvent in organic synthesis. Breathing sodium borohydride can irritate the lungs causing coughing andor shortness of breath. After the addition is complete, the suspension is stirred for 30 minutes. Previously 1, we demonstrated that sodium borohydride can be also successfully employed for the reductive amination of carbonyl compounds by inorganic acid salts of ammonia and primary and secondary amines. Since the reaction rate for the reduction of iminium ions is much faster than for ketones or even aldehydes, the reductive amination can be carried out as a onepot procedure by introducing the reducing agent into a mixture of the amine and carbonyl compound.
Show this safety data sheet to the doctor in attendance. Our msds database is an easy to use, do it yourself tool, and best of all. Jul 14, 2011 i need to find a method to analyse the clearance of sodium cyanoborohydride throughout a process we believe it has been cleared but we are strugglinf to find an assay that will show this with high sensitivity. There is no warranty of accuracy or completeness of any information contained herein. Sodium borohydride cas 16940662 106371 merck millipore. The bound cyanoborohydride can be utilized as a versatile reducing agent for the reductive amination of carbonyl compounds and reduction of imines. Add 2aa and sodium cyanoborohydride mixes to each sample. Biotage mpcyanoborohydride is a macroporous polymer supported cyanoborohydride. This solution is filtered, heated to reflux on a steam bath and then dioxan 150ml. Biotage mpcyanoborohydride is a macroporous polymer supported cyanoborohydride, which is a solidsupported equivalent of tetraalkylammonium cyanoborohydride. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Filtration followed by vacuum removal of thf gives white solid sodium cyanoborohydride. Very high exposure to sodium borohydride may affect the nervous system.
Add 2aa and sodium cyanoborohydride mixes to each sample add 50 l of the 2aa dye followed by 50 l of sodium cyanoborohydride to each of the samples and controls. Sodium cyanoborohydride is the chemical compound with the formula na b h 3 cn. The 2aa labeling kit contains two sets of the following reagents 15 samples per kit supplied in glass ampoules sealed under pure nitrogen. The cyanoborohydride offers five times milder reduction.
Eye contact in the case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Sigmaaldrich offers a number of sodium cyanoborohydride products. Synthesis of nabh3 cn and related compounds thiokol. The cyanoborohydride offers five times milder reduction compared to borohydride in.
Sodium borohydride is widely used for the reduction of carbonylcontaining compounds to alcohols. W336 red sodium cyanoborohydride t, f w336 ox sodium periodate f w336 acid cabinet conc. Lane marker sample buffer, nonreducing, 5x, 5 ml, 0. Carbonyl groups such as aldehydes, ketones, and glyox.
The entry for will lead you to the original literature which details the synthesis of sodium cyanoborohydride and also the typical procedures used in reductive amination or other selective reductions. It can be prepared either by treating sodium cyanide with borane, or by reacting sodium borohydride with mercuryii cyanide. Sodium cyanoborohydride cas 25895607 scbt santa cruz. Sodium cyanoborohydride, sodium cyanotrihydroborate. Safety data sheet according to federal register vol. I am considering using sodium cyanoborohydride in a reductive nalkylation of a polysaccharide, and want to know how to handle it safely and dispose of the nitrile byproduct.
Purification of sodium cyanoborohydride 2 sodium cyanoborohydride 10g is dissolved in thf 80ml and 1 m methanolic hydrochloric acid is added until ph reaches 9. For the love of physics walter lewin may 16, 2011 duration. Sds of sodium cyanoborohydride, safety data sheets, cas. Safety data sheet creation date 29jan2010 revision date 17jan2018 revision number 4 1. How can i handle and dispose sodium cyanoborohydride. Important if the denatured protein precipitates, discard the sample and restart this process. Safety data sheet 112030 sodium triacetoxyborohydride. Acid destabilizes the sodium cyanoborohydride which results in an activated state for kinetics and equilibrium. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database.
Sodium cyanoborohydride is a very hygroscopic material and contact with water or moist air should be avoided. When using scbh in reductive amination, the ph must be maintained in the range of 68. Structural integrity no detectable sodium cyanoborohydride, sodium cyanotrihydroborate, molecular formula. It is a colourless salt, but commercial samples can appear tan. Wear protective glovesprotective clothingeye protectionface protection. Sodium triacetoxyborohydride, 97%, acros organics fisher.
Articles of sodium cyanoborohydride are included as well. Read the safety data sheet for 1 m sodium cyanoborohydride. However, we make no warranty of merchantability or any other warranty. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the. Left over reagent which contains sodium cyanoborohydride has to be disposed separately in a clearly marked bottle due to its toxicity see msds. Contact can severely irritate and burn the skin and eyes with possible eye damage. Immediately flush eyes with running water for at least 15 minutes, keeping eyelids open. Examples include reduction of a,bunsaturated carbonyl compounds to the corresponding. It is widely used in organic synthesis for the reduction of imines. Ludgertag 2aa 2aminobenzoic acid glycan labeling kit. Number of samples typically, up to 15 separate analytical samples per set of labeling reagents. Breathing sodium borohydride can irritate the nose and throat. Specifically, compared with the small molecule sodium cyanoborohydride, it is reported that. Sodium cyanoborohydride can be made by reacting equimolar quantities of sodium borohydride and hydrogen cyanide in thf.
Why sodium cyanoborohydride reactivity depends on ph. Sodium cyanoborohydride reagent grade, 95% sigmaaldrich. The solution is then poured with stirring into dioxan 250ml. Sodium tetrahydroborate powder recommended use laboratory chemicals. Skin contact wash off immediately with plenty of water for at least 15 minutes.
Please read the material safety data sheets msds for all chemicals used see appendix 2. Reason for citation sodium borohydride is on the hazardous substance list. Reductive methylation of protein amino groups with formaldehyde and sodium cyanoborohydride is shown to give up to 25% yield of ncyanomethyl ch2cn product. Suitable samples any purified glycans with free reducing termini can be labeled. Sodium cyanoborohydride is preferred as a reducing agent over sodium borohydride as the latter will also reduce the reactive aldehydes to hydroxyls at the same time as reducing the schiffs bases. And it is usually used as a mild reducing agent which converts imines to amines. Sodium cyanoborohydride cas 25895607 msds guidechem. Sodium cyanoborohydride cas 25895607 818053 emd millipore. The submitter has found that use of sodium borohydride instead of sodium cyanoborohydride in the present procedure results in the almost exclusive formation of cyclohexanol with less than 3% of basic material. Allowing the material to come into contact with water or moist air may decompose the material and could lead to a violent reaction or explosion due to the release of hydrogen gas. Find msds or sds, a coa, data sheets and more information. Working with hazardous chemicals organic syntheses.